Journal: Stem Cells and Cloning : Advances and Applications
Article Title: Characterization of neural stemness status through the neurogenesis process for bone marrow mesenchymal stem cells
doi: 10.2147/SCCAA.S94545
Figure Lengend Snippet: After 72 hours of culturing mouse MSCs in MEM +20% FBS, note that some of the cells became adherent and began to elongate as revealed under inverted microscope. Notes: ( A )10×; ( B )20×. Abbreviations: MSCs, mesenchymal stem cells; MEM, minimum essential medium; FBS, fetal bovine serum.
Article Snippet: There were four different formulas: 1) MEM media with 5% FBS alone, 2) MEM supplemented with 50 ng/mL epidermal growth factors (EGFs; United States Biological, MA, USA; E3374-11), 3) MEM supplemented with 50 ng/mL basic fibroblast growth factors (b-FGFs) (United States Biological; F4210-20M1), and 4) MEM with 50 ng/mL for each b-FGF and EGF.
Techniques: Inverted Microscopy